Andor Spinning Disk


 

 

The Andor Spinning Disk Confocal Microscope, located on the 5th floor of the Rosenstiel Center, is equipped with both spinning disk confocal and epifluorescence modes, as well as a Mosaic laser photobleaching/photoactivation device. The system is set up to maximize speed for sequential or simultaneous detection of multiple fluorophores and includes:

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

  • Microscope: An upright microscope (Nikon Ni-E) with an XY motorized stage and a piezo Z drive. The objective turret includes 10x and 20x air, 40x, 60x and 100x oil, and 60x water dipping objectives.
  • Fluorescence illumination: Five laser lines (445 nm – 40 mW, 488 nm – 100 mW, 515 nm, 50 mW, 561 nm – 100 mW, 640 nm, 100 mW), as well as a Lumencor light engine with six LED lines (405, 440, 488, 515, 561 and 640 nm).
  • Spinning Disk: A Yokogawa CSU-W1 spinning disk head with a 50 mm pinhole disk as well as a bypass option.
  • EMCCD cameras: Two Andor iXon 897U EMCCD cameras (512x512 16 mm pixels), for use in sequential imaging with single emission or quad emission filters in a motorized wheel, or for simultaneous two-color imaging via an Andor TuCam device for green/red imaging.
  • sCMOS camera: For epifluorescence imaging, an Andor Zyla sCMOS camera (2560x2160 6.5 mm pixels). Equipped for sequential imaging with single emission or quad emission filters in a motorized wheel.
  • Photoillumination/bleaching: A 405 nm (450 mW) laser illuminating a Mosaic digital mirror illuminator, capable of instantaneously bleaching or photoactivating multiple areas of any geometry.
  • Software: Online and offline licenses for Nikon Elements AR, with deconvolution module.