Brandeis EM Documentation
Frequently Asked Questions for Brandeis CryoEM
EM room conditions
Here you can find some information about operating instructions, calibration
data info, etc..
Temperature and Humidity - Data Plots for F30 and F20 Rooms
Since Oct. 15, 2008.
Since April 27, 2010
Operating Morgagni 268 Microscope
A brief operating manual for Morgagni 268.
AMT CCD Imaging
An operating manual for AMT CCD equipped on Morgagni 268.
A step-by-step guide to align CM12 and setup low-dose imaging conditions.
Ultrascan 1000 CCD with CM12
Some information about this Gatan US1000 CCD and calibration results with CM12.
CM12 Quiz. See how much you know about CM12.
TF20 & TF30 Related
Work with Cameras on F20
Information about Falcon, Orius and US4000 on F20.
FEI Eagle 4K CCD on TF20
Some basic information about the FEL Eagle 4K CCD installed on TF20.
Cryo Holder Insertion for TF30
A pdf file describing the correct steps to inserting cryo holder into F30.
F30 Operator's Reminding List
F30 Operator's Reminding list.
Operating F20/F30 for Single Particle with SerialEM
A very brief, but fairly complete checking list for F20 or F30 operation for
single particle data collection using SerialEM.
Procedures for Tecnai TF30 Shutdown and Startup
How to shutdown and startup TF30 properly.
GIF Distortion Field Correction
GIF Distortion Field Correction - procedure and results.
Image Distortion Field off GIF Camera Since Aug 08, 2008
GIF Image Distortion Field Since Aug 08, 2008
Image Distortion Field of GIF Camera Since Dec 01, 2011
GIF Image Distortion Field Since Dec. 01, 2011
Image Distortion Field of GIF Camera Since K2 install Aug 01, 2012
GIF Image Distortion Field Since K2 camera Installation at Aug. 01, 2012
Beyond Tilting Series
A protocol to use SerialEM for single particle data collection. On CCD and film. It also includes
random conical tilt pair data collecting.
SerialEM HowTo: Positioning X,Y
SerialEM HowTo: Positioning Z
SerialEM HowTo: Vary Defocus Value in Fly
SerialEM HowTo: Fix Scripting Beam Blank
SerialEM Help Files
The latest SerialEM help file can be found as SerialEM.chm.
There is also one in html and
The *.chm is always up to date, as it is a direct link to the source in ftp archive.
Some FEI documents in pdf format
Tecnai on-line help -- Alignment
Tecnai on-line help -- Userinterface
Tecnai on-line help -- Options
Tecnai on-line help -- Freeware
Some General Information
Cryo Grid Storage Record
A table recording the ownership of the cylinder and tubes for our auto-filling,
string-free cryo grids storage system. It has brief description for the system
we designed for cryo grids storage.
Negative Stain Procedure
A guide to make 1% UA solution from stock and the steps to negatively staining an EM grid.
A guide to make uranyl acetate solution for negative staining.
work-Study Student Handbook
This handbook contains all the information about the microscope lab setup, maintaining
procedures, resource of the supplies, instruction for making negative stain solutions, etc..