Using Falcon II camera on Tecnai F30

Chen Xu

$BrandeisEM: ~/emdoc/articles-xml/FlaconII-on-F30/article.xml 1 2013-10-10 12:08:37 xuchen Exp$

Falcon II/Orius camera system is now moved from Tecnai F20 to Tecnai F30. As you probably knew this already, Falcon II has back-thinned chip sensor, comparing to Falcon I. The Tecnai software is also upgraded to version 4.5.1 now. This version of Tecnai software supports intermediate frame (movie) mode. SerialEM was also implemented with new features to support the movie mode. With current FEI hardware and software setup, the movie mode supporting is still limited, but it is better than nothing.

You can also get pdf version of this document here.


Table of Contents
1 Switching between Normal Image Mode and Intermediate Frame Mode
2 Prepare Gain Reference Files from Tecnai Interface
3 Intermediate Frame Mode with SerialEM

1 Switching between Normal Image Mode and Intermediate Frame Mode

This is something new.

There is now an executable to run so we can switch between the two modes. Here is the window.

It is important to switch to "Normal acquisition mode" in the end of your session. In fact, when you exit from SerialEM, it will pop up a message window to remind you of this.