Image Distortion Field of GIF Camera Since K2 install Aug 01, 2012

Chen Xu

$BrandeisEM: emdoc-xml/en_US.ISO8859-1/articles/GIF-idf-Jan15_2013/article.xml 1 2013-03-01 02:41:42 xuchen Exp$

GIF lenses add another field to your final image collected on GIF CCD camera. Correction of the distortion field has been proven to be neccesary. Following the procedure provided by David Mastronarde, I did the correction for our TF30(Tecnai v4.0.3, DM v1.8.3, SerialEM v3.2.3 and beta)

The specimen used for this testing data is a 2/2 quantifoil grid with 10nm Au particles dispersed on it densely. I have more confidence with this rigid sample, compared to plastic sections.

K2 Summit camera installation involves GIF tube higheit change. Therefore, a new distortion field mesaurement is needed. I did it for magnifications of 13.5kX and 22.5kX.


    gif135k_01Aug2012.idf for mag 13.5kX
    gif225k_01Aug2012.idf for mag 22.5kX
    

1 Distortion Field Measuring Data Collection

The data condition:


Date = Jan 14, 2013
H.T. = 300kV
Gun lens = 2
Ext.Vol. = 3950V
C2 aperture = 100um
Obj. aperture =  50um

2 Analysis of the Data

The complete screen output for 13.5kX processing is as below.


[xuchen@oxford ~/GIF-Distortion-Jan10-2013]$ finddistort -bin 2 -pat dp-gif135k.st gif135k -box 40 -ind 2

 RO image file on unit   1 : dp-gif135k.st     Size=      16390 K

 Number of columns, rows, sections .....    1024    1024       8
 Map mode ..............................    1   (16-bit integer)           
 Start cols, rows, sects, grid x,y,z ...    0     0     0    1024   1024      8
 Pixel spacing (Angstroms)..............   19.99      19.99      19.99    
 Cell angles ...........................   90.000   90.000   90.000
 Fast, medium, slow axes ...............    X    Y    Z
 Origin on x,y,z .......................    0.000       0.000       0.000    
 Minimum density .......................   870.00                               
 Maximum density .......................   16334.                               
 Mean density ..........................   7497.2                               
 tilt angles (original,current) ........   0.0   0.0   0.0   0.0   0.0   0.0
 Space group,# extra bytes,idtype,lens .        0     5120        0        0

     2 Titles :
SerialEM: Camera on TF30 0:GIF 1:K2 Summit 2:Orius      14-Jan-13  15:49:04    
    Tilt axis angle = -26.0, binning = 2  spot = 4  camera = 0                 

 pair           0           1 , deltas   477.2770       10.10000    
 pair           2           3 , deltas   323.7150       326.3550    
 pair           4           5 , deltas   32.43200       492.6750    
 pair           6           7 , deltas  -290.4110       319.4500    

 FORMATTED  new  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif135k.rawxf
 Solving for          26  by          26  =         676  field positions
        4846  rows of data,       38431  entries
 condition #   754.4031       742.993822957211     
 Mean and max residual in fit:   0.254  0.814
 Mean and max change in field:    1.3685   8.2421

 FORMATTED  new  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif135k.nosidf

 FORMATTED  new  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif135k.udxf
Shift changes:   0.81   0.38   0.71   1.29   0.06   1.27  -0.38   0.63
 iteration           2    mean change in shift   1.134173    
        4846  rows of data,       38631  entries
 condition #   735.2344       734.008653979646     
 Mean and max residual in fit:   0.300  0.922
 Mean and max change in field:    0.0459   0.2463

 FORMATTED  new  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif135k.nosidf

 FORMATTED  new  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif135k.udxf
Shift changes:   0.01   0.00   0.01   0.02  -0.02  -0.01   0.00   0.01
 iteration           3    mean change in shift  1.5204709E-02
        4846  rows of data,       38633  entries
 condition #   715.5957       714.429419290499     
 Mean and max residual in fit:   0.306  0.930
 Mean and max change in field:    0.0039   0.0181

 FORMATTED  new  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif135k.nosidf

 FORMATTED  new  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif135k.udxf
Shift changes:   0.00   0.00   0.00   0.00   0.00   0.00   0.00   0.00
 iteration           4    mean change in shift  1.7256646E-03
        4846  rows of data,       38633  entries
 condition #   735.3134       714.396273671806     
 Mean and max residual in fit:   0.307  0.930
 Mean and max change in field:    0.0004   0.0021

 FORMATTED  new  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif135k.nosidf

 FORMATTED  new  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif135k.udxf
Shift changes:   0.00   0.00   0.00   0.00   0.00   0.00   0.00   0.00
 iteration           5    mean change in shift  5.0020218E-04
        4846  rows of data,       38633  entries
 condition #   715.6835       714.421406380709     
 Mean and max residual in fit:   0.307  0.931
 Mean and max change in field:    0.0001   0.0004

 FORMATTED  new  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif135k.nosidf

 FORMATTED  new  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif135k.udxf
Shift changes:   0.00   0.00   0.00   0.00   0.00   0.00   0.00   0.00
 iteration           6    mean change in shift  0.0000000E+00
        4846  rows of data,       38633  entries
 condition #   715.6364       734.161197468163     
 Mean and max residual in fit:   0.307  0.931
 Mean and max change in field:    0.0000   0.0000

 FORMATTED  new  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif135k.nosidf

 FORMATTED  new  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif135k.udxf
Shift changes:   0.00   0.00   0.00   0.00   0.00   0.00   0.00   0.00
 iteration           7    mean change in shift  0.0000000E+00
        4846  rows of data,       38633  entries
 condition #   735.4537       714.373599424215     
 Mean and max residual in fit:   0.307  0.931
 De-stretch transformation:  0.999757 -0.000507 -0.000561  1.000017

 FORMATTED  new  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif135k.patch
 Embedded transformation:  0.999986 -0.000008 -0.000008  0.999979

 FORMATTED  new  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif135k.idf

[xuchen@oxford ~/GIF-Distortion-Jan10-2013]$ matchrotpairs -d gif135k.nosidf -b 2 gif135kst1.st gif135kst2.st rp-gif135k.xf
Finding the best matched pair of views in the two series:
              (Type Ctrl-C to end search)
A 1  B 1  rotation -90  difference 256699 *
A 1  B 1  rotation +90  difference 851968 
Views in best pair: A 1  B 1
(data only has 1 section anyway)

[xuchen@oxford ~/GIF-Distortion-Jan10-2013]$ finddistort -bin 2 -pat -usexf -str rp-gif135k.xf dp-gif135k.st gif135k -box 40 -ind 2

 RO image file on unit   1 : dp-gif135k.st     Size=      16390 K

 Number of columns, rows, sections .....    1024    1024       8
 Map mode ..............................    1   (16-bit integer)           
 Start cols, rows, sects, grid x,y,z ...    0     0     0    1024   1024      8
 Pixel spacing (Angstroms)..............   19.99      19.99      19.99    
 Cell angles ...........................   90.000   90.000   90.000
 Fast, medium, slow axes ...............    X    Y    Z
 Origin on x,y,z .......................    0.000       0.000       0.000    
 Minimum density .......................   870.00                               
 Maximum density .......................   16334.                               
 Mean density ..........................   7497.2                               
 tilt angles (original,current) ........   0.0   0.0   0.0   0.0   0.0   0.0
 Space group,# extra bytes,idtype,lens .        0     5120        0        0

     2 Titles :
SerialEM: Camera on TF30 0:GIF 1:K2 Summit 2:Orius      14-Jan-13  15:49:04    
    Tilt axis angle = -26.0, binning = 2  spot = 4  camera = 0                 


 FORMATTED  ro  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif135k.rawxf
 pair           0           1 , deltas   477.2770       10.10000    
 pair           2           3 , deltas   323.7150       326.3550    
 pair           4           5 , deltas   32.43200       492.6750    
 pair           6           7 , deltas  -290.4110       319.4500    
 Solving for          26  by          26  =         676  field positions
        4846  rows of data,       38431  entries
 condition #   754.4031       742.993822957211     
 Mean and max residual in fit:   0.254  0.814
 Mean and max change in field:    1.3685   8.2421

 FORMATTED  new  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif135k.nosidf

 FORMATTED  new  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif135k.udxf
Shift changes:   0.81   0.38   0.71   1.29   0.06   1.27  -0.38   0.63
 iteration           2    mean change in shift   1.134173    
        4846  rows of data,       38631  entries
 condition #   735.2344       734.008653979646     
 Mean and max residual in fit:   0.300  0.922
 Mean and max change in field:    0.0459   0.2463

 FORMATTED  new  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif135k.nosidf

 FORMATTED  new  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif135k.udxf
Shift changes:   0.01   0.00   0.01   0.02  -0.02  -0.01   0.00   0.01
 iteration           3    mean change in shift  1.5204709E-02
        4846  rows of data,       38633  entries
 condition #   715.5957       714.429419290499     
 Mean and max residual in fit:   0.306  0.930
 Mean and max change in field:    0.0039   0.0181

 FORMATTED  new  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif135k.nosidf

 FORMATTED  new  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif135k.udxf
Shift changes:   0.00   0.00   0.00   0.00   0.00   0.00   0.00   0.00
 iteration           4    mean change in shift  1.7256646E-03
        4846  rows of data,       38633  entries
 condition #   735.3134       714.396273671806     
 Mean and max residual in fit:   0.307  0.930
 Mean and max change in field:    0.0004   0.0021

 FORMATTED  new  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif135k.nosidf

 FORMATTED  new  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif135k.udxf
Shift changes:   0.00   0.00   0.00   0.00   0.00   0.00   0.00   0.00
 iteration           5    mean change in shift  5.0020218E-04
        4846  rows of data,       38633  entries
 condition #   715.6835       714.421406380709     
 Mean and max residual in fit:   0.307  0.931
 Mean and max change in field:    0.0001   0.0004

 FORMATTED  new  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif135k.nosidf

 FORMATTED  new  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif135k.udxf
Shift changes:   0.00   0.00   0.00   0.00   0.00   0.00   0.00   0.00
 iteration           6    mean change in shift  0.0000000E+00
        4846  rows of data,       38633  entries
 condition #   715.6364       734.161197468163     
 Mean and max residual in fit:   0.307  0.931
 Mean and max change in field:    0.0000   0.0000

 FORMATTED  new  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif135k.nosidf

 FORMATTED  new  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif135k.udxf
Shift changes:   0.00   0.00   0.00   0.00   0.00   0.00   0.00   0.00
 iteration           7    mean change in shift  0.0000000E+00
        4846  rows of data,       38633  entries
 condition #   735.4537       714.373599424215     
 Mean and max residual in fit:   0.307  0.931
 De-stretch transformation:  0.999757 -0.000507 -0.000561  1.000017

 FORMATTED  ro  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/rp-gif135k.xf
Pair has rotation =  -89.84, mag = 0.9834, stretch = 1.0374 on   24.7 deg axis
Mean mag = 1.0016, using mag = 0.9818 and stretch = 1.0374
 No-mag transformation:  0.016707  0.988282 -1.012047 -0.011238
True rotation =  -89.84, underlying stretch =  1.0092 on  -65.2 deg axis
 Stretch transformation:  0.994101 -0.006986 -0.006986  1.005983
 Implied no-mag transformation:  0.016707  0.988282 -1.012047 -0.011239

 FORMATTED  new  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif135k.patch
 Embedded transformation:  0.994087 -0.006994 -0.006994  1.005962

 FORMATTED  new  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif135k.idf
 

The complete screen output for 22.5kX processing is as below.


[xuchen@oxford ~/GIF-Distortion-Jan10-2013]$ finddistort -bin 2 -pat dp-gif225k.st gif225k -box 56 -ind 2

 RO image file on unit   1 : dp-gif225k.st     Size=      16390 K

 Number of columns, rows, sections .....    1024    1024       8
 Map mode ..............................    1   (16-bit integer)           
 Start cols, rows, sects, grid x,y,z ...    0     0     0    1024   1024      8
 Pixel spacing (Angstroms)..............   11.12      11.12      11.12    
 Cell angles ...........................   90.000   90.000   90.000
 Fast, medium, slow axes ...............    X    Y    Z
 Origin on x,y,z .......................    0.000       0.000       0.000    
 Minimum density .......................   838.00                               
 Maximum density .......................   14075.                               
 Mean density ..........................   6269.5                               
 tilt angles (original,current) ........   0.0   0.0   0.0   0.0   0.0   0.0
 Space group,# extra bytes,idtype,lens .        0     5120        0        0

     2 Titles :
SerialEM: Camera on TF30 0:GIF 1:K2 Summit 2:Orius      14-Jan-13  15:54:06    
    Tilt axis angle = -11.9, binning = 2  spot = 4  camera = 0                 

 pair           0           1 , deltas   435.5000       29.84300    
 pair           2           3 , deltas   326.3600       347.7110    
 pair           4           5 , deltas   29.69300       510.7970    
 pair           6           7 , deltas  -273.7150       327.5860    

 FORMATTED  new  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif225k.rawxf
 Solving for          25  by          25  =         625  field positions
        4741  rows of data,       37437  entries
 condition #   903.1486       897.035982706462     
 Mean and max residual in fit:   0.220  0.906
 Mean and max change in field:    1.2609   8.2089

 FORMATTED  new  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif225k.nosidf

 FORMATTED  new  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif225k.udxf
Shift changes:   0.42   0.21   0.41   0.90  -0.18   1.19  -0.32   0.49
 iteration           2    mean change in shift  0.8663639    
        4741  rows of data,       37504  entries
 condition #   912.3472       931.261787535582     
 Mean and max residual in fit:   0.257  0.948
 Mean and max change in field:    0.0496   0.3074

 FORMATTED  new  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif225k.nosidf

 FORMATTED  new  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif225k.udxf
Shift changes:   0.00   0.00   0.02   0.02  -0.01   0.02   0.00   0.00
 iteration           3    mean change in shift  1.9432150E-02
        4741  rows of data,       37504  entries
 condition #   911.9879       910.174271689720     
 Mean and max residual in fit:   0.263  0.953
 Mean and max change in field:    0.0052   0.0186

 FORMATTED  new  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif225k.nosidf

 FORMATTED  new  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif225k.udxf
Shift changes:   0.00   0.00   0.00   0.00   0.00   0.00   0.00   0.00
 iteration           4    mean change in shift  1.4942370E-03
        4741  rows of data,       37504  entries
 condition #   909.4825       910.145490033507     
 Mean and max residual in fit:   0.264  0.953
 Mean and max change in field:    0.0004   0.0012

 FORMATTED  new  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif225k.nosidf

 FORMATTED  new  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif225k.udxf
Shift changes:   0.00   0.00   0.00   0.00   0.00   0.00   0.00   0.00
 iteration           5    mean change in shift  0.0000000E+00
        4741  rows of data,       37504  entries
 condition #   912.6151       910.146914834609     
 Mean and max residual in fit:   0.264  0.953
 Mean and max change in field:    0.0000   0.0001

 FORMATTED  new  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif225k.nosidf

 FORMATTED  new  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif225k.udxf
Shift changes:   0.00   0.00   0.00   0.00   0.00   0.00   0.00   0.00
 iteration           6    mean change in shift  0.0000000E+00
        4741  rows of data,       37504  entries
 condition #   908.8071       910.145931247637     
 Mean and max residual in fit:   0.264  0.953
 De-stretch transformation:  0.999758 -0.000345 -0.000544  0.999857

 FORMATTED  new  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif225k.patch
 Embedded transformation:  0.999993  0.000002  0.000002  0.999978

 FORMATTED  new  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif225k.idf

[xuchen@oxford ~/GIF-Distortion-Jan10-2013]$ matchrotpairs -d gif225k.nosidf -b 2 gif225kst1.st gif225kst2.st rp-gif225k.xf
Finding the best matched pair of views in the two series:
              (Type Ctrl-C to end search)
A 1  B 1  rotation -90  difference 198521 *
A 1  B 1  rotation +90  difference 673009 
Views in best pair: A 1  B 1
(data only has 1 section each anyway)

[xuchen@oxford ~/GIF-Distortion-Jan10-2013]$ finddistort -bin 2 -pat -usexf -str rp-gif225k.xf dp-gif225k.st gif225k -box 56 -ind 2

 RO image file on unit   1 : dp-gif225k.st     Size=      16390 K

 Number of columns, rows, sections .....    1024    1024       8
 Map mode ..............................    1   (16-bit integer)           
 Start cols, rows, sects, grid x,y,z ...    0     0     0    1024   1024      8
 Pixel spacing (Angstroms)..............   11.12      11.12      11.12    
 Cell angles ...........................   90.000   90.000   90.000
 Fast, medium, slow axes ...............    X    Y    Z
 Origin on x,y,z .......................    0.000       0.000       0.000    
 Minimum density .......................   838.00                               
 Maximum density .......................   14075.                               
 Mean density ..........................   6269.5                               
 tilt angles (original,current) ........   0.0   0.0   0.0   0.0   0.0   0.0
 Space group,# extra bytes,idtype,lens .        0     5120        0        0

     2 Titles :
SerialEM: Camera on TF30 0:GIF 1:K2 Summit 2:Orius      14-Jan-13  15:54:06    
    Tilt axis angle = -11.9, binning = 2  spot = 4  camera = 0                 


 FORMATTED  ro  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif225k.rawxf
 pair           0           1 , deltas   435.5000       29.84300    
 pair           2           3 , deltas   326.3600       347.7110    
 pair           4           5 , deltas   29.69300       510.7970    
 pair           6           7 , deltas  -273.7150       327.5860    
 Solving for          25  by          25  =         625  field positions
        4741  rows of data,       37437  entries
 condition #   903.1486       897.035982706462     
 Mean and max residual in fit:   0.220  0.906
 Mean and max change in field:    1.2609   8.2089

 FORMATTED  new  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif225k.nosidf

 FORMATTED  new  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif225k.udxf
Shift changes:   0.42   0.21   0.41   0.90  -0.18   1.19  -0.32   0.49
 iteration           2    mean change in shift  0.8663639    
        4741  rows of data,       37504  entries
 condition #   912.3472       931.261787535582     
 Mean and max residual in fit:   0.257  0.948
 Mean and max change in field:    0.0496   0.3074

 FORMATTED  new  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif225k.nosidf

 FORMATTED  new  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif225k.udxf
Shift changes:   0.00   0.00   0.02   0.02  -0.01   0.02   0.00   0.00
 iteration           3    mean change in shift  1.9432150E-02
        4741  rows of data,       37504  entries
 condition #   911.9879       910.174271689720     
 Mean and max residual in fit:   0.263  0.953
 Mean and max change in field:    0.0052   0.0186

 FORMATTED  new  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif225k.nosidf

 FORMATTED  new  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif225k.udxf
Shift changes:   0.00   0.00   0.00   0.00   0.00   0.00   0.00   0.00
 iteration           4    mean change in shift  1.4942370E-03
        4741  rows of data,       37504  entries
 condition #   909.4825       910.145490033507     
 Mean and max residual in fit:   0.264  0.953
 Mean and max change in field:    0.0004   0.0012

 FORMATTED  new  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif225k.nosidf

 FORMATTED  new  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif225k.udxf
Shift changes:   0.00   0.00   0.00   0.00   0.00   0.00   0.00   0.00
 iteration           5    mean change in shift  0.0000000E+00
        4741  rows of data,       37504  entries
 condition #   912.6151       910.146914834609     
 Mean and max residual in fit:   0.264  0.953
 Mean and max change in field:    0.0000   0.0001

 FORMATTED  new  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif225k.nosidf

 FORMATTED  new  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif225k.udxf
Shift changes:   0.00   0.00   0.00   0.00   0.00   0.00   0.00   0.00
 iteration           6    mean change in shift  0.0000000E+00
        4741  rows of data,       37504  entries
 condition #   908.8071       910.145931247637     
 Mean and max residual in fit:   0.264  0.953
 De-stretch transformation:  0.999758 -0.000345 -0.000544  0.999857

 FORMATTED  ro  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/rp-gif225k.xf
Pair has rotation =  -89.88, mag = 1.0187, stretch = 0.9613 on  -82.2 deg axis
Mean mag = 0.9988, using mag = 1.0199 and stretch = 0.9613
 No-mag transformation:  0.007465  0.981209 -1.019176 -0.003181
True rotation =  -89.88, underlying stretch =  1.0099 on  -82.1 deg axis
 Stretch transformation:  0.990557 -0.002671 -0.002671  1.009540
 Implied no-mag transformation:  0.007484  0.981209 -1.019176 -0.003199

 FORMATTED  new  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif225k.patch
 Embedded transformation:  0.990551 -0.002669 -0.002669  1.009517

 FORMATTED  new  file opened: /gusr/xuchen/linux/GIF-Distortion-Jan10-2013/gif225k.idf