Uranly Acetate: Preparation and Packaging for Lab Use

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1 Preparing 4% Uranyl Acetate Stock Solution (total volume 100 ml):

Materials:

  1. Uranyl acetate powder

  2. balance that can be moved to a fume hood

  3. Hot plate

  4. milli-Q filtered water

  5. weighing dish

  6. brown 200ml stock bottle

  7. Whatman #1 filter paper

  8. glass funnel

  9. 100 ml volumatric flask

  10. Latex Gloves, lab coat, protective mask

  11. stir bar

  12. stirring plate

Note: Once glassware is contaminated with heavy metal stains it should not be used to prepare other chemical solutions)

Procedure:

  1. Have balance set up in the fume hood.

  2. Heat to near boiling approximately 125ml of milli-Q water.

  3. Weigh out 4 grams of uranyl acetate powder into a 200ml flask taking care not to breath the dust.

  4. Add approximately 98ml of hot water to the flask.

  5. Stir on stir plate until dissolved(this will take a while and small percentage of salt will not go into solution).

  6. Cool solution down to room temperature.

  7. Filt 4% stock solution into brown 200ml bottle(UA solutions are sensitive to light) and cab tightly and label. This stock solution can be store for months at room temperature.

  8. Rinse contaminated glassware into a waste bottle for uranyl acetate solution and store separate from laboratory glassware. Solid waste that is contaminated with uranyl acetate should be disposed of in a solid waste container set aside for radioactive wastes.


2 Preparing 1% and 2% Uranyl Acetate Solutions:

Materials:

  1. Graduated Cylinder

  2. 4% Uranyl Acetate Stock Solutions

  3. glass distilled water

Procedure:

  1. To make 1% UA solution, add 1 part of 4% stock to 3 part of water. Filter this solution into a brown storage bottle using a 2 micron filter fitted onto a syringe.

  2. To make 2% US solution, add 1 part of 4% stock to 1 part of water. Filter as in step 1 above.

  3. The stains can be stored at room temperature and used directly from these bottles or aliquots of the stain can be transferred into smaller containers(such as microfuge tubes) for individual use to avoid cross contamination.

  4. Clean up, being sure to dispose of all the contaminated wastes properly.