11 Pixelsize and Magnification Calibration

The default factory magnification calibration might not be good enough if you need a accurate value for size measurements. Finally, I did a mag and pixel size calibration for each mag. The specimen used for this calibration are 463nm grating replica grid for mag of 8900x and lower, and homemade Tropomysin para-crystals in negative stain for 8900x and higher.

Mag_ScreenDown(x) Mag_ScreenUp(x) Mag_OnCCD(x) Pixelsize@1280x1024(nm)
880 1000 1,188.47 21.19
1100 1300 1,519.85 15.79
1400 1600 2,029.25 11.83
1800 2000 2,400.90 10.00
2200 2500 3,229.22 7.43
2800 3200 3,796.33 6.32
3500 4000 4,896.05 4.90
4400 5000 5,982.03 4.01
5600 6300 9,940.55 2.41
7100 8000 10,971.83 2.19
8900 10000 12,895.61 1.87
11000 13000 16,204.35 1.48
14000 16000 20,102.28 1.19
18000 20000 25,108.86 0.956
22000 25000 30,571.14 0.785
28000 32000 40,205.91 0.597
36000 40000 51,524.05 0.466
44000 50000 61,768.10 0.389
56000 63000 83,240.51 0.288
71000 80000 100,392.04 0.239
89000 100000 124,046.58 0.194
110000 125000 154,389.87 0.156
140000 160000 197,574.68 0.122
180000 200000 232,283.54 0.103