Magnification Table for Gatan US4000 CCD on Tecnai F20

Chen Xu

$BrandeisEM: ~/emdoc/articles-xml/mag-table-US4000-on-F20/article.xml 1 2013-09-18 02:39:36 xuchen Exp$

Since Sept 16, 2013, FEI Falcon Camera & Gatan Orius CCD system was taken off from Tecnai F20 to be put on Tecnai F30, after Gatan K2 Summit Camera departure. The only camera left available on Tecnai F20 is Gatan US4000 CCD.

Gatan US4000 CCD level was raised from below FEI Falcon Camera to right after column, the camera to film ratio changed. Therefore, the pixelsize (on specimen level) is also changed. For both TIA and SerialEM, the pixelsize needs to be recalibrated.

This document lists a table for calibration of exact pixelsizes on CCD at different magnifications.

You can also get pdf version of this document here.


Table of Contents
1 Mag & Pixel Size Calibration table

1 Mag & Pixel Size Calibration table

The calibration was done using standard 0.463um distance grating grid.

Mag Index Mag_ScreenUp(X) Mag_OnCCD(X) PixelSize@4096(nm)
1 21 27.2 551.188
2 28 36.6 409.295
3 38 51.1 293.328
4 56 74.4 201.540
5 75 99.3 151.074
6 97 129.5 115.834
7 120 163 92.298
8 170 225 66.777
9 220 297 49.926
10 330 452 33.180
11 420 576 26.039
12 550 753 19.723
13 800 1087 13.680
14 1100 2058 10.238
15 1500 2046 7.302
16 2100 2840 5.283
17 1700 2327 6.447
18 2500 3461 4.337
19 3500 4748 3.159
20 5000 6955 2.157
21 6500 8934 1.681
22 7800 10563 1.420
23 9600 13100 1.145
24 11500 15697 0.954
25 14500 20083 0.745
26 19000 26781 0.560
27 25000 33609 0.446
28 29000 40123 0.374
29 50000 67591 0.222
30 62000 83701 0.179
31 80000 108799 0.138
32 100000 135471 0.111
33 150000 201556 0.0744
34 200000 270859 0.0554
35 240000 335252 0.0447
36 280000 395620 0.0379