12 Prepare Gain Reference Files

This is done with proper beam and NO specimen. Either specimen rod is in retracted position or there is a hole or broken area that is large than the CCD area. As we mainly use SerialEM for this preparation task, this is done from SerialEMCameraPrepare Gain Reference.

For the new generation direct detector camera such as Gatan K2 Summit camera and FEI Falcon camera, the procedure to prepare gain reference is more complicated. Therefore, it is perhaps not a bad idea to use the native controlling software to prepare the gain reference. You can ask SerialEM to use that. This is done by SerialEMCamera → uncheck Normalize here.