16 Eucentricity, without wobbling the stage if possible.

If possible, try not to use procedure that involves stage wobble. The main reason is that wobbling the stage will likely introduce LN2 bubbling again in the dewar of 626 side-entry cryo holder. The quickest way is perhaps to run Z_byVmacro in Low-Dose mode.

Example 1. Z_byV


MacroName Z_byV
##############################
# Z_byV.txt
# by Chen Xu, Feb 8, 2013
##############################
#
# a macro to adjust the eccentric center using beam tilted pairs.
# It uses Autofocus to measure the focus and adjust Z instead.
#
# assume the Eucentric Focus has been calibrated for the mag you use.

#==================================
# remember the starting focus for V
#==================================
GoToLowDoseArea V
ReportFocus
DEF = $ReportedValue1

#====================
# set objective lens 
#====================
SetEucentricFocus
NormalizeLenses 2
Delay 1

#===========
# Adjust Z
#===========
Loop 2
Autofocus -1 1
ReportAutofocus 
t = -1 * $reportedValue1
MoveStage 0 0 $t
echo --> Z moved $t micron 
EndLoop

#==================================
# set back original defocus of View 
#==================================
SetStandardFocus $DEF