Operating Morgagni 268

Chen Xu

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This is a quick check list for user who is new the Morgagni microscope. Quite different from the CM12 or 420T interface, Morgagni 268 comes with a very nice computer control interface which is very similar to that of Tecnai series of microscopes. This documentation is aim to help you use this new interface to control the scope.

For details of operating the scope and all other information, please refer to the hard copy of original "Operation Manual" that came with the scope.

A bottom mount model of AMT CCD camera is installed on Morgagni 268. This is 1k X 1k camera with a nice software control. We no longer keep an camera in the chamber, but one can get a camera for CM12/420T to use it in Morgagni if you do want to take images on films. The documentation of operating this AMT CCD camera can be found separately as "AMT-CCD-imaging".

You can also get pdf version of this document here.


Table of Contents
1 Check logbook to see if there have been any problems
2 Log in to Windows 2000 computer
3 Start Morgagni User Interface
4 Check Vacuum
5 H.T. on
6 Set filament to saturation and check gun tilt
7 Check C2 aperture mechanical position and C2 stigmatism
8 Check specimen holder & load grid
9 Insert specimen holder into column
10 Specimen position can be "remembered" by the stage
11 Put in objective aperture and center it
12 Check objective lens stigmatism
13 Finishing ...

1 Check logbook to see if there have been any problems

It is always good and necessary to check the logbook. You can find some very useful information from it.

Important: Usually the scope is kept on all the time. However, there are situations that the scope was shutdown. When you come to the Morgagni room and find it is off, please ASK before you turn it back ON!


2 Log in to Windows 2000 computer

We share a common userid "emlab" on both Morgagni and CCD computer. Ask Chen for the password if you don't know.

This computer usually is not connected to network.


3 Start Morgagni User Interface

After login, Morgagni User Interface can be started as one of the following ways:


4 Check Vacuum

Check the vacuum status from "vacuum" and "vacuum overview" modules.


5 H.T. on

High Tension can only be turned on from Panel "H.T" button. It needs to be pressed longer than 0.5 second. A very brief hit won't turn it on due to safety protection reason.


6 Set filament to saturation and check gun tilt


7 Check C2 aperture mechanical position and C2 stigmatism

Two ways to adjust C2 mechanical position, you can use either one.

There are two ways to check and adjust the C2 stigmatism.


8 Check specimen holder & load grid

This is rather important. If you see any problem with any of the holders, report to the manager immediately. Otherwise, you could be the one countable for the problem. Several things about the holder need to be checked before use.

Gently mount the specimen grid on the its position. Use the tool pin to open and close the clamping device.


9 Insert specimen holder into column

Important: Be careful! Only at this step might you damage the scope or specimen holder mechanically. Be sure that you understand what you are doing. Should you feel any uncomfortable or confusion about the action, please stop and seek help.


10 Specimen position can be "remembered" by the stage

The stage movement is also sent to two laser sensors which located on top of two small motors. Although this is not a CompuStage system, the positions can be stored.


11 Put in objective aperture and center it

It is very important to know what size of the obj. aperture you are using. And the position of the aperture will affect the obj. lens stigmatism. Here are the steps.


12 Check objective lens stigmatism


13 Finishing ...