Shuttle Device for Use in a Shared Commercial NMR Instrument Version II

We have developed a device that can be installed in our shared commercial 500 MHz NMR instrument, to move a sample from its usual position at the center of the magnet out to a lower-field position above the magnet, and back, with a minimum round-trip time of 100 msec. This permits us to insert a low-field interval into any pulse sequence, that can be used to study relaxation or cross-polarization rates at the lower field. At these lower fields, heteronuclear dipolar relaxation between spins, which produces NOE's (nuclear Overhauser Effects), is enhanced compared to other competing relaxation mechanisms. Major uses of this device are: checking and extending NMR-based studies of protein dynamics, including ligand dynamics; and studies of nucleic acid backbone geometry based on proton-to-31P NOE. Currently we are testing the apparatus with short DNA duplexes. We also expect to participate in collaborations with others at Brandeis, using this device.

Dr. Redfield is a Member of the National Academy of Sciences and a Fellow of the American Academy of Arts and Sciences.

Selected Publications:

"Conformational and Dynamic Differences Between N-ras P21 Bound to GTPgS and to GMPPNP as Studied by NMR" (J. S. Hu & A. G. Redfield) Biochemistry 36, 5045-5052 (1997).[abstract]

"Development of a Field Cycling NMR System for PQR Detection in Biopolymers" (D. Ivanov & A. G. Redfield) Zeitschrift fur Naturforschung 53a, 269-272 (1998). [abstract]

"Development of a Field-Cycling NMR Method to Study Quadrupolar Nuclei in Biological Systems." Ph.D. Thesis, Dmitri Ivanov, January, 2001.

"Boron-11 pure quadrupole resonance investigation of peptide boronic acid inhibitors bound to alpha-lytic protease", Ivanov D, Bachovchin WW, Redfield AG, Biochemistry, 41:1587-90. (2002) [abstract]

"Field-cycling method with central transition readout for pure quadrupole resonance detection in dilute systems", Ivanov D, Redfield AG., J Magn Reson., 166:19-27 (2004). [abstract]

"High-resolution field-cycling NMR studies of a DNA octamer as a probe of phosphodiester dynamics and comparison with computer simulation", Roberts MF, Cui Q, Turner CJ, Case DA, Redfield AG. Biochemistry, 43:3637-50 (2004). [abstract]

 

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Last update: October 1, 2004. E-mail comments or questions to the webmaster.