Our lab is interested in the signal transduction that regulates organization of the cytoskeleton.
One focus of our research is the molecular mechanism of cell division. Cell division can either be symmetric, generating two identical cells, or asymmetric, generating cells with different characteristics or developmental fates. Asymmetric cell divisions play crucial roles in development and are particularly important role in maintaining stem cell populations. Furthermore, cell division failure results in doubling of the genetic material, which can lead to aneuploidy and cancer.
Another area of our research interest is in the mechanisms that regulate Rho-type GTPases, which are key regulators of cell division. Misregulation of Rho activity is often linked to human diseases; however, spatial and temporal regulation of Rho activity in the cell is poorly understood.
We use the genetically tractable budding yeast as a simple model system. Budding yeast has been a leading model for studying cell division in general and specifically for studying the cytoskeletal events necessary for asymmetric division. Because the genes and processes we study are highly conserved, understanding fundamental mechanisms of cell division in yeast is highly relevant for understanding human diseases.
List of Publications:
Mechanisms for concentrating Rho1 during cytokinesis. Yoshida S, Bartolini S, Pellman D. Genes and Development (2009) Genes Dev. 2009 Apr 1;23(7):810-23.[abstract]
Symmetry breaking: scaffold plays matchmaker for polarity signaling proteins. Atkins BD, Yoshida S, Pellman D. Current Biology (2008) Dec 23;18(24) [abstract]
Filling the GAP between Cell Cycle and Polarity. Yoshida S, Pellman D. EMBO Reports (2008) Jan;9(1) [abstract]
Yeast formins Bni1 and Bnr1 utilize different modes of cortical interaction during the assembly of actin cables. Buttery S*, Yoshida S*, Pellman D. Molecular Biology of the Cell (2007) May;18(5) (*Equal contribution) [abstract]
Polo-like kinase Cdc5 controls the local activation of Rho1 to promote cytokinesis. Yoshida S, Kono K, Lowery DM, Bartolini S, Yaffe MB, Ohya Y, Pellman D. Science (2006) Jul 7;313(5783) [abstract]
MEN signaling: daughter bound pole must escape her mother to be fully active. Yoshida S, Guillet M, Pellman D. Developmental Cell (2005) Aug;9(2) [abstract]
Ras recruits mitotic exit regulator Lte1 to the bud cortex in budding yeast. Yoshida S, Ichihashi R, Toh-e A. Journal of Cell Biology (2003) Jun 9;161(5) [abstract]
Mitotic exit network controls the localization of Cdc14 to the spindle pole body in Saccharomyces cerevisiae. Yoshida S, Asakawa K, Toh-e A. Current Biology (2002) Jun 4;12(11) [abstract]
Budding yeast Cdc5 phosphorylates Net1 and assists Cdc14 release from the nucleolus. Yoshida S, Toh-e A. Biochem Biophys Res Commun. (2002) Jun 14;294(3) [abstract]
Last edit: July 31, 2009