We are studying the function of HINT (histidine triad nucleotide
binding) protein, a small regulatory protein which occurs
in all forms of life. The mammalian protein has been cloned,
expressed, and its crystal structure has been obtained.
Function is being determined using knock-outs.
When the work load of the heart is increased or when it
becomes hypoxic, it responds by increasing the coronary
blood flow. It does this by releasing the vasodilator adenosine
which is formed by activation of AMP hydrolysis. The AMPase
responsible for producing adenosine in heart is 5'-nucleotidase
N-I. The regulatory properties of the enzyme are under investigation.
Selected Publications:
Matsushita K, Morrell CN, Cambien B, Yang SX, Yamakuchi
M, Bao C, Hara MR, Quick RA, Cao W, O'Rourke B, Lowenstein
JM, Pevsner J, Wagner DD, Lowenstein CJ. (2003) Nitric oxide
regulates exocytosis by S-nitrosylation of N-ethylmaleimide-sensitive
factor. Cell. 2003 Oct 17;115:139-50. [abstract]
Saura M, Zagora C, McMillan A, Quick RA, Hohenadl CA, Lowenstein
JM, Lowenstein CJ. (1999) An Antiviral Mechanism of Nitric
Oxide: Inhibition of a Viral Protease. Immunity 10:21-28.
[abstract]
Pawelczyk T, Lowenstein JM. (1997) Effect of different
species of sphingomyelin on phospholipase C 1 activity.
Biochimie 79: 741-748. [abstract]
Ghosh S, Pawelczyk T, Lowenstein JM. (1997) Phospholipase
C isoforms delta 1 and delta 3 from human fibroblasts. High-yield
expression in Escherichia coli, simple purification, and
properties. Protein Expr Purif 9:262-78. [abstract]
Brenner C, Garrison P, Gilmour J, Peisach D, Ringe D, Petsko
GA, Lowenstein JM. (1997) Crystal structures of HINT demonstrate
that histidine triad proteins are GalT-related nucleotide-binding
proteins. Nat Struct Biol 4 :231-8 . [abstract]
Gilmour J, Liang N, Lowenstein JM. (1997) Isolation, cloning
and characterization of a low-molecular-mass purine nucleoside-
and nucleotide-binding protein. Biochem J 326
( Pt 2) :471-7 . [abstract]
Richard EA, Ghosh S, Lowenstein JM, Lisman JE. (1997) Ca2+/calmodulin-binding
peptides block phototransduction in Limulus ventral photoreceptors:
evidence for direct inhibition of phospholipase C. Proc
Natl Acad Sci U S A 94:14095-9. [abstract]
View Complete Publication List on PubMed:
John Lowenstein
Last reviewed: January 30, 2007. E-mail comments
or questions to the webmaster.
Top
of Page
| Life Science
Faculty | Home
