Facilities

The equipment we work with

Our lab has a full range of state-of-the-art equipment for protein purification, fluorometry and fluorescent and light microscopy. We also share a number of facilities with related labs in the Rosenstiel center, and the biochemistry, microarray, and confocal microscopy facilities at Brandeis.

To read more about a particular piece of equipment or facility, click on one of the links below.

ÄKTA FPLC

This FPLC from Amersham Pharmacia is used for purification of a range of proteins with various gel filtration and anion/cation exchange columns. Our FPLC is contained in its own refrigeration unit and is controlled externally through the "Unicorn" software system. We also have the associated automatic fractionator to simplify eluate collection.

PDF ÄKTA FPLC specifications

PTI fluorometer

We currently use two PTI QM-4/2003 spectrofluorometers to carry out kinetics assays for actin assembly and disassembly under a variety of conditions. Reactions are manipulated using the Felix software system.

HTML Specifications from PTI

Zeiss Microscope

We use a Zeiss Axioskop fluoresence microscope for imaging live and fixed cells, using a Hamamatsu Orca ER cooled CCD camera and Openlab imaging software (including deconvolution package).

PDF Axioskop40 specifications

Centrifuges

Our lab has a large centrifuge core containing multiple high speed centrifuges. Large samples may be spun in either of two Sorvall RC-5B centrifuges, while larger G forces can be applied using our two ultracentrifuges (Beckman Coulter Optima LE-80K and an older Beckman L8-70). We also have a Beckman TL-100 tabletop ultracentrifuge which can be used for high speed applications such as sedimenting actin filaments.

PDF Optima LE-80K specifications

HTML Beckman TL-100 specifications

Fermentor

Large quantities of yeast cells may be grown to specific optical densities using our fermentor.

Our fermentor

Brandeis facilities

Brandeis University has a number of facilities that are shared among labs and specialized equipment which may be used by any of the biology, biochemistry, chemistry and biophysics labs on campus.

The Biochemistry Core Facility (DNAlab)

The Confocal Imaging Lab

Electron Microscopy - W. M. Keck Institute for Cellular Visualization

List of Shared Facilities Available to Life Sciences Laboratories

Please notify the webmaster of any broken links, missing graphics or incorrect information. All contents copyright 2000-2007, Goode Research Laboratory, Brandeis University. All rights reserved. Last modified February 9, 2007. This site was designed and created by Jacqueline Hendries.