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Protocols

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Doing that thing we do

Fluorometry assays Actin assembly kinetics Actin filament elongation					Freezing and lysing yeast Freezing Red Star Yeast Yeast lysis with coffee grinder Yeast lysis with Waring blender [Word document] Yeast lysis with Waring blender [Quicktime video]
Protein purification Yeast actin purification AAP purification (20g) Arp2/3 complex (HA-TEV) purification					Other assays Yeast actin assembly assay (timepoints) Yeast permeabilized cell assay Fast and Efficient Yeast Genomic DNA Prep

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