Recent Publications

 

2014

J Wang, X Han, CCL Wong, H Cheng, A Aslanian, T Xu, P Leavis, H Roder, L Hedstrom, JR Yates, III, and A Kashina. Arginylation of intact proteins in vivo involves a novel type of Arg linkage to amino acid side chains.  Nat. Chem. Biol, 21, 331-7 (2014). abstract


117.  SK Gorla, NN McNair, G Yang, S Gao, M Hu, VR  Jala, B Haribabu, B Striepen, GD Cuny, JR Mead and L Hedstrom. Validation of IMP dehydrogenase inhibitors in a mouse model of cryptosporidiosis. Antimicrob. Agents Chemother. 58, 1603-14 (2014). abstract


2013


SK Gorla, M Kavitha, M Zhang , JEW Chin*, X Liu, B Striepen, M Makowska-Grzyska, Y Kim, A Joachimiak, L Hedstrom and GD Cuny. Optimization of benzoxazole-based inhibitors of Cryptosporidium parvum inosine 5’-monophosphate dehydrogenase. J. Med. Chem. 56, 4028-43 (2013). abstract


CR Johnson, SK Gorla,  M Kavitha, M Zhang, X Liu, B Striepen, JR Mead, GD Cuny  and L Hedstrom.  Phthalazinone Inhibitors of

Inosine-5’-Monophosphate Dehydrogenase from Cryptosporidium parvum. Biorg. Med. Chem. Lett. 23, 1004-7 (2013). abstract


2012


MJC Long, DR Gollapalli and L Hedstrom.  Inhibitor Mediated Protein Degradation.  Chemistry & Biology 19, 629-37 (2012). Nature News & Views, abstract


SK Gorla, M Kavitha, M Zhang, X Liu, L Sharling, DR Gollapalli, B Striepen, L Hedstrom and GD Cuny.  Selective and potent urea inhibitors of Cryptosporidium parvum inosine 5’-monophosphate dehydrogenase. J. Med. Chem. 55, 7759-71 (2012). abstract


Makowska-Grzyska, M.; Kim, Y.; W., R.; Wilton, R.; Gollapalli, D.R.; *Wang, X.K.; Zhang, R.; Jedrzejczak, R.; Mack, J.C.; Maltseva, N.; Mulligan, R.; Binkowski, T.A; Gornicki, P.; Kuhn, M.; Anderson, W.F.; Hedstrom, L and Joachimiak, A. Bacillus anthracis IMP Dehydrogenase in Action:  Structures of Apoenzyme and in Complex with Substrate and Product.  Biochemistry 51, 6148-63 (2012). abstract


Y Gao, MJC Long, J Shi, L Hedstrom and B Xu.  Using supramolecular hydrogel to discover the interactions between proteins and molecular nanofibers of small molecules.  Chem. Commun. (Camb) 48, 8404-6 (2012). abstract


MJC Long and L Hedstrom.  Mushroom Tyrosinase Oxidizes Tyrosine-rich Sequences, Allowing Selective Protein Functionalization.  ChemBiochem 13, 1818-25 (2012). abstract


L. Hedstrom. The dynamic determinants of reaction specificity in the IMPDH/GMPR family of (β/α)8 barrel enzymes.  Critical Reviews In Biochemistry & Molecular Biology 47, 250-63 (2012). abstract


S Kirubakaran, SK Gorla, L Sharling, M Zhang, X Liu, SS Ray, IS MacPherson, B Striepen, L Hedstrom and GD Cuny.  Structure-activity relationship study of selective benzimidazole-based inhibitors of Cryptosporidium parvum IMPDH.  Bioorg Med Chem Lett. 22, 1985-8 (2012). abstract


2011


X. E. Sun, B.G. Hansen, and L. Hedstrom. Kinetically Controlled Drug Resistance: How Penicillium brevicompactum Survives Mycophenolic Acid. J. Biol. Chem. 286, 40595-600 (2011). abstract


B.G. Hansen, X.E. Sun, H.J. Genee,C.S. Kaas, J.B. Nielsen,U.H. Mortensen, J.C. Frisvad, and L. Hedstrom. Adaptive evolution of drug targets in producer and non-producer organisms. Biochemical Journal 441, 219-26 (2011). abstract


I.S. MacPherson, J.S. Temme, S. Habeshian, K. Felczak, K. Pankiewicz, L. Hedstrom and I.J. Krauss. Multivalent glycocluster design through directed evolution.  Angew. Chem. Int. Ed. 50, 11238-42 (2011). Hot Paper. abstract


T.V. Riera, L. Zheng, H.R. Josephine, D. Min, W. Yang and L. Hedstrom.  Allosteric activation via kinetic control: Potassium accelerates a conformational change in IMP dehydrogenase. Biochemistry 50, 8508-18 (2011). abstract


G.C. Patton, P. Stenmark, D.R. Gollapalli, R. Sevastik,P. Kursula,S. Flodin, H. Schuler, C.T. Swales, H. Eklund, F. Himo, P.

Nordlund and L. Hedstrom. Cofactor mobility determines reaction outcome in the IMPDH/GMPR (β/α)8 barrel enzymes. Nat. Chem. Biol.7, 950-8 (2011). abstract


M.J.C. Long, Y. Pan, H.-C. Lin, L. Hedstrom, and B. Xu.  Cell Compatible Trimethoprim (TMP)-Decorated Iron Oxide Nanoparticles Bind Dihydrofolate Reductase (DHFR) for Magnetically Modulating Focal Adhesion of Mammalian Cells. J. Am. Chem. Soc. 133, 10006-9 (2011).  Faculty of 1000. abstract


Y. Pan, M.J.C. Long, X. Li, J. Shi, L. Hedstrom and B. Xu. Glutathione (GSH)-Modified Magnetic Nanoparticles to Bind Glutathione-S-transferase (GST) Fusion Proteins. Chemical Science 2, 945-948 (2011). 


  1. L.Hedstrom, G. Liechti, J.B. Goldberg and D.R. Gollapalli. The antibiotic potential of prokaryotic IMP dehydrogenase inhibitors.  Current Medicinal Chemistry 18, 1909-18 (2011). abstract


2010


B.C. Hoefler, D.R. Gollapalli, and L. Hedstrom. Specific Biotinylation of IMP Dehydrogenase. Bioorg. Med. Chem. Lett. 21, 1363-1365 (2010). abstract


H.R. Josephine, K.R. Ravichandran and L. Hedstrom.  The Cys319 loop modulates the transition between dehydrogenase and hydrolase conformations in IMP dehydrogenase. Biochemistry 49, 10674-81 (2010). abstract


B.E. Mansfield, H.N. Oltean, B.G. Oliver, S.E. Leyde, L. Hedstrom and T.C. White.  Azole Drug Import Requires a Transporter in Candida albicans and Other Pathogenic Fungi.  PLoS Pathogens 6, e1001126.  Faculty of 1000. abstract


D.R. Gollapalli, I.S. MacPherson, G. Liechti, J.B. Goldberg,and L. Hedstrom. Structural Determinants of Inhibitor Selectivity in Prokaryotic IMP Dehydrogenases, Chemistry and Biology 17, 1084-1091 (2010).  featured in SciBX 3(44); doi:10.1038/scibx.2010.1327. abstract


L. Sharling, Lisa, X. Liu, D.R. Gollapalli, S. Maurya, L. Hedstrom and B. Striepen.  A screening pipeline for antiparasitic agents targeting Cryptosporidium inosine monophosphate dehydrogenase. PLoS Neglected Tropical Diseases, 4, e794 (2010). abstract


X.E. Sun, L. Sharling, M. Muthalagi, D.G. Mudeppa, K.W. Pankiewicz, K.  Felczak, P.K. Rathod, J. Mead, B. Striepen and L. Hedstrom.  Prodrug activation by Cryptosporidium thymidine kinase. J. Biol. Chem. 285, 15,916-15,922 (2010).  abstract


I.S. MacPherson, S. Kirubakaran, S.K. Gorla, T.V. Riera, J.A. D'Aquino, M. Zhang, G.D. Cuny and L. Hedstrom.  The Structural Basis of Cryptosporidium-Specific IMP Dehydrogenase Inhibitor Selectivity. J. Am. Chem. Soc., 132, 1230-1231 (2010). abstract


2009


Maurya, Sushil; Gollapalli, Deviprasad; Kirubakaran, Sivapriya; Zhang, Minjia; *Johnson, Corey ; *Benjamin, Nicole; Hedstrom, Lizbeth and Cuny, Gregory D. Triazole inhibitors of Cryptosporidium parvum inosine 5’-monophosphate dehydrogenase, J. Med. Chem. 52, 4623-4630 (2009). abstract


Sazonova, Irina Y.; McNamee, Rachel A. ; Houng, Aiilyan K.; King, Sarah M.; Hedstrom, Lizbeth  and Reed, Guy L. Reprogrammed Streptokinases Develop Fibrin-targeting and Dissolve Blood

Clots with More Potency than Tissue Plasminogen Activator, J. Thromb. Haemost. 7, 1321-1328 (2009). abstract


Hedstrom, L.  IMP dehydrogenase: structure, mechanism and inhibition.  Chemical Reviews 109, 2903-2928 (2009). abstract


2008


Mortimer, S.E.; Xu, D, McGrew, D.; Hamaguchi, N.; Lim, H.C.; Bowne, S.J.; Daiger, S.P. and  Hedstrom, L. IMP dehydrogenase type 1 associates with polyribosomes translating rhodopsin mRNA. J. Biol. Chem. 283, 36254-36360 (2008). abstract


Min, Donghong; Josephine, Helen R.; Li, Hongzhi; Lakner, Clemens; MacPherson, Iain S.; Naylor, Gavin. J.P.; Swofford, David; Hedstrom,, Lizbeth and Yang, Wei.  An Enzymatic Atavist Revealed in Dual Pathways for Water Activation.  PLoS Biology 6, e206 (2008). featured article in PLoS Biology, C&E News and Physorg.comabstract


Riera, T.V.; Wang, W.; Josephine, H.R. and Hedstrom, L.  A Kinetic alignment of orthologous IMP dehydrogenases.  Biochemistry 47, 8689-96 (2008).  abstract


Umejiego, Nwakaso N.;  Gollapalli, Deviprasad;  Sharling, Lisa; *Volftsun, Anna; *Lu, Jennifer; *Benjamin, Nicole N.; *Stroupe, Adam H.;  Riera1, Thomas V.; Striepen,, Boris; Hedstrom, Lizbeth.  Targeting a prokaryotic protein in a eukaryotic pathogen:  identification of lead compounds against Cryptosporidiosis. Chem. Biol. 15, 70-77 (2008). featured in Science Editor's Choice, Faculty of 1000, Voice of America, Science Daily and Cryptosporidium Blog. abstract