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Post-Doctoral Fellow


Education
2006 - MS Chemistry, Department of Biophysical Chemistry, University of Groningen, Groningen, The Netherlands
20011 - PhD Chemistry, Department of Biophysical Chemistry, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Groningen, The Netherlands

Contact
Email: ottenr @ brandeis.edu
Office: Volen 443
Dept. of Biochemistry, MS 009
Brandeis University
415 South Street
Waltham, MA 02454, USA
Phone (Lab): (781) 736-2326
Website:Personal Webpage

Renee Otten


Research Interests

Over the past decade(s) it has been become increasingly evident that both a high-resolution structure and a detailed insight in its molecular dynamics are of critical importance to understand the function of a biological macromolecule. Nuclear magnetic resonance (NMR) spectroscopy is one of the most powerful techniques to study protein dynamics over a wide range of time scales and, moreover, can provide such information for many different nuclei with atomic resolution. I am interested in the development and application of NMR methodology to study protein structure/dynamics, with the goal to elucidate the intricate relationship between protein structure/dynamics and enzyme catalysis.


Publications

Padua RA, Sun Y*, Marko I, Pitsawong W, Stiller JB, Otten R, Kern D Mechanism of activating mutations and allosteric drug inhibition of the phosphatase SHP2 Nature Communications (2018) 9, 4507

Pitsawong W*, Buosi V*, Otten R*, Agafonov RV*, Zorba A, Kern N, Kutter S, Kern G, Padua RA, Meniche X, Kern D Dynamics of human protein kinase Aurora A linked to drug selectivity. eLIFE (2018) 7, e36656

Otten R*, Liu L*, Kenner LR, Clarkson MW, Mavor D, Tawfik DS, Kern D, Fraser JS Rescue of conformational dynamics in enzyme catalysis by directed evolution. Nature Communications 2018) 9, 1314

Niklasson M, Otten R, Ahlner A, Andresen C, Schlagnitweit J, Petzhold K, Lundstroem PComprehensive analysis of NMR data using advanced line shape fitting. J Biomol NMR (2017) 69(2):93-99

Chakrabarti KS, Agafonov RV, Pontiggia F, Otten R, Higgins MK, Schertler GF, Oprian DD, Kern D. Conformational Selection in a Protein-Protein Interaction Revealed by Dynamic Pathway Analysis. Cell Reports. (2016) Jan 5;14(1):32-42. doi: 10.1016/j.celrep.2015.12.010. Epub 2015 Dec 24. PMID: 26725117.

Oktaviani NA, Risor MW, Lee YH, Megens RP, de Jong DH, Otten R, Scheek RM, Enghild JJ, Nielsen NC, Ikegami T, Mulder FAA Optimized co-solute paramagnetic relaxation enhancement for the rapid NMR analysis of a highly fibrillogenic peptide. J. Biomol. NMR (2015) 62(2): 129-142

Wilson C, Agafonov RV, Hoemberger M, Kutter S, Zorba A, Halpin J, Buosi V, Otten R, Waterman D, Theobald DL, Kern D. Using ancient protein kinases to unravel a modern cancer drug's mechanism. Science (2015) 347(6224):882-886

Hass MA, Liu WM, Agafonov R.V., Otten R, Phung LA, Schilder JT, Kern D, Ubbink M. A minor conformation of a lanthanide tag on adenylate kinase characterized by paramagnetic relaxation dispersion NMR spectroscopy. J Biomol NMR (2015) 61(2):123-36

Kerns SJ, Agafonov R.V., Cho YJ, Pontiggia F, Otten R, Pachov DV, Kutter S, Phung LA, Murphy PN, Thai V, Alber T, Hagan MF, Kern D. The energy landscape of adenylate kinase during catalysis. Nature Structural & Molecular Biology (2015) 22(2):124-31

Maeno SJ, Sindhikara D, Hirata F, Otten R, Dahlquist FW, Yokoyama S, Akasaka K, Mulder FAA, Kitahara R Cavity as a Source of Conformational Fluctuation and High-Energy State: High-Pressure NMR Study of a Cavity-Enlarged Mutant of T4 Lysozyme. Biophys. J. (2015) 108(1): 133-145

Chu BCH, Otten, Krewulak KD, Mulder FAA, Vogel HJ The solution structure, binding properties, and dynamics of the bacterial siderophore-binding Protein FepB. J Biol Chem (2014) 289(42): 29219-29234

Agafonov R.V., Wilson C, Otten R, Buosi V, Kern D. Energetic dissection of Gleevec's selectivity toward human tyrosine kinases. Nature Structural & Molecular Biology (2014) 21(10):848-53

Meniche X, Otten R, Siegrist MS, Baer CE, Murphy KC, Bertozzi CR, Sassetti CM Subpolar addition of new cell wall is directed by DivIVA in mycobacteria. PNAS (2014) 111(31: E3243-51

Wood K, Gallat FX, Otten R, van Heel AJ, Lethier M, van Eijck L, Moulin M, Haertlein M, Weik M, Mulder FAA Protein surface and core dynamics show concerted hydration-dependent activation. Angew Chem Int Ed Engl (2013) 52(2): 665-8

Stockbridge RB, Lim HH, Otten R, Williams C, Shane T, Weinberg Z, Miller C Fluoride resistance and transport by riboswitch-controlled CLC antiporters. PNAS (2012) 18; 109(38): 15289-94

Wood K, Paz A, Dijkstra K, Scheek RM, Otten R, Silman I, Sussman JL, Mulder FAA Backbone and side chain NMR assignments for the intrinsically disordered cytoplasmic domain of human neuroligin-3. Biomol NMR Assign (2012) 6: 15-18

Karasawa, A.; Erkens, G. B.; Berntsson, R. P. A.; Otten, R.; Schuurman-Wolters, G. K.; Mulder, F. A. A. & Poolman, B. Cystathionine beta-Synthase (CBS) Domains 1 and 2 Fulfill Different Roles in Ionic Strength Sensing of the ATP-binding Cassette (ABC) Transporter OpuA. J. Biol. Chem. (2011) 286 (43): 37280-37291

Mulder, F. A. A.; Otten, R. & Scheek, R. M. Origin and removal of mixed-phase artifacts in gradient sensitivity enhanced heteronuclear single quantum correlation spectra. J. Biomol. NMR (2011), 51 (1-2): 199-207

Meinema, A. C.; Laba, J; K.; Hapsari, R. A.; Otten, R.; Mulder, F. A. A.; Kralt, A.; van den Bogaart, G.; Lusk, C. P.; Poolman, B. & Veenhoff, L. M. Long Unfolded Linkers Facilitate Membrane Protein Import Through the Nuclear Pore Complex. Science (2011) 333 (6038): 90-93

Wood, K.; Paz, A.; Dijkstra, K.; Scheek, R. M.; Otten, R.; Silman, I.; Sussman, J. L. & Mulder, F. A. A. Backbone and side chain NMR assignments for the intrinsically disordered cytoplasmic domain of human neuroligin-3 Biomol. NMR Assign. (2011) x (x): xx - xx

Oktaviani, N. A.; Otten, R.; Dijkstra, K.; Scheek, R. M.; Thulin, E.; Akke, M. & Mulder, F. A. A. 100% Complete Assignment of Non-Labile 1H, 13C, and 15N Signals for Calcium-Loaded calbindin D9kP43G Biomol. NMR Assign. (2011) 5 (1): 79 - 84

Otten, R. Protein structure and dynamics by NMR: synergy between biochemistry and pulse sequence design PhD thesis (2011) Department of Biophysical Chemistry, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Groningen, The Netherlands

Otten, R.; Villali, J.; Kern, D. & Mulder, F. A. A. Probing Microsecond Time Scale Dynamics in Proteins by Methyl 1H Carr-Purcell-Meiboom-Gill Relaxation Dispersion NMR Measurements. Application to Activation of the Signaling Protein NtrC′ J. Am. Chem. Soc. (2010) 132 (47): 17004 - 17014

Otten, R.; Chu, B.; Krewulak, K. D.; Vogel, H. J. & Mulder, F. A. A. Comprehensive and Cost-Effective NMR Spectroscopy of Methyl Groups in Large Proteins J. Am. Chem. Soc. (2010) 132 (9): 2952 - 2960

Otten, R.*; Wood, K.* & Mulder, F. A. A. Comprehensive Determination of 3JHNHα for Unfolded Proteins Using 13C′-Resolved Spin-Echo Difference Spectroscopy J. Biomol. NMR (2009) 45 (4): 343 - 349 (*both authors contributed equally to this work)

Louhivuori, M.; Otten, R.; Salminen, T. & Annila, A. Evidence of Molecular Alignment Fluctuations in Aqueous Dilute Liquid Crystalline Media J. Biomol. NMR, (2007) 39 (2): 141 - 152

Louhivuori, M.; Otten, R.; Lindorff-Larsen, K. & Annila, A. Conformational Fluctuations Affect Protein Alignment in Dilute Liquid Crystal Media J. Am. Chem. Soc. (2006) 128 (13): 4371 - 4376

Otten, R.; van Lune, F. S.; Dijkstra, K. & Scheek, R. M. Letter to the Editor: 1H, 13C, and 15N Resonance Assignments of the Phosphorylated Enzyme IIB of the Mannitol-Specific Phophoenolpyruvate-Dependent Phosphotransferase System of Escherichia coli J. Biomol. NMR (2004) 30 (4): 461 - 642

Publication List on PubMed