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Post-Doctoral Fellow


Education
2006 - MS Chemistry, Department of Biophysical Chemistry, University of Groningen, Groningen, The Netherlands
20011 - PhD Chemistry, Department of Biophysical Chemistry, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Groningen, The Netherlands

Contact
Email: ottenr @ brandeis.edu
Office: Volen 443
Dept. of Biochemistry, MS 009
Brandeis University
415 South Street
Waltham, MA 02454, USA
Phone (Lab): (781) 736-2326
Website:Personal Webpage

Renee Otten


Research Interests

Over the past decade(s) it has been become increasingly evident that both a high-resolution structure and a detailed insight in its molecular dynamics are of critical importance to understand the function of a biological macromolecule. Nuclear magnetic resonance (NMR) spectroscopy is one of the most powerful techniques to study protein dynamics over a wide range of time scales and, moreover, can provide such information for many different nuclei with atomic resolution. I am interested in the development and application of NMR methodology to study protein structure/dynamics, with the goal to elucidate the intricate relationship between protein structure/dynamics and enzyme catalysis.


Publications

Karasawa, A.; Erkens, G. B.; Berntsson, R. P. A.; Otten, R.; Schuurman-Wolters, G. K.; Mulder, F. A. A. & Poolman, B. Cystathionine beta-Synthase (CBS) Domains 1 and 2 Fulfill Different Roles in Ionic Strength Sensing of the ATP-binding Cassette (ABC) Transporter OpuA. J. Biol. Chem. (2011) 286 (43): 37280-37291

Mulder, F. A. A.; Otten, R. & Scheek, R. M. Origin and removal of mixed-phase artifacts in gradient sensitivity enhanced heteronuclear single quantum correlation spectra. J. Biomol. NMR (2011), 51 (1-2): 199-207

Meinema, A. C.; Laba, J; K.; Hapsari, R. A.; Otten, R.; Mulder, F. A. A.; Kralt, A.; van den Bogaart, G.; Lusk, C. P.; Poolman, B. & Veenhoff, L. M. Long Unfolded Linkers Facilitate Membrane Protein Import Through the Nuclear Pore Complex. Science (2011) 333 (6038): 90-93

Wood, K.; Paz, A.; Dijkstra, K.; Scheek, R. M.; Otten, R.; Silman, I.; Sussman, J. L. & Mulder, F. A. A. Backbone and side chain NMR assignments for the intrinsically disordered cytoplasmic domain of human neuroligin-3 Biomol. NMR Assign. (2011) x (x): xx - xx

Oktaviani, N. A.; Otten, R.; Dijkstra, K.; Scheek, R. M.; Thulin, E.; Akke, M. & Mulder, F. A. A. 100% Complete Assignment of Non-Labile 1H, 13C, and 15N Signals for Calcium-Loaded calbindin D9kP43G Biomol. NMR Assign. (2011) 5 (1): 79 - 84

Otten, R. Protein structure and dynamics by NMR: synergy between biochemistry and pulse sequence design PhD thesis (2011) Department of Biophysical Chemistry, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Groningen, The Netherlands

Otten, R.; Villali, J.; Kern, D. & Mulder, F. A. A. Probing Microsecond Time Scale Dynamics in Proteins by Methyl 1H Carr-Purcell-Meiboom-Gill Relaxation Dispersion NMR Measurements. Application to Activation of the Signaling Protein NtrC′ J. Am. Chem. Soc. (2010) 132 (47): 17004 - 17014

Otten, R.; Chu, B.; Krewulak, K. D.; Vogel, H. J. & Mulder, F. A. A. Comprehensive and Cost-Effective NMR Spectroscopy of Methyl Groups in Large Proteins J. Am. Chem. Soc. (2010) 132 (9): 2952 - 2960

Otten, R.*; Wood, K.* & Mulder, F. A. A. Comprehensive Determination of 3JHNHα for Unfolded Proteins Using 13C′-Resolved Spin-Echo Difference Spectroscopy J. Biomol. NMR (2009) 45 (4): 343 - 349 (*both authors contributed equally to this work)

Louhivuori, M.; Otten, R.; Salminen, T. & Annila, A. Evidence of Molecular Alignment Fluctuations in Aqueous Dilute Liquid Crystalline Media J. Biomol. NMR, (2007) 39 (2): 141 - 152

Louhivuori, M.; Otten, R.; Lindorff-Larsen, K. & Annila, A. Conformational Fluctuations Affect Protein Alignment in Dilute Liquid Crystal Media J. Am. Chem. Soc. (2006) 128 (13): 4371 - 4376

Otten, R.; van Lune, F. S.; Dijkstra, K. & Scheek, R. M. Letter to the Editor: 1H, 13C, and 15N Resonance Assignments of the Phosphorylated Enzyme IIB of the Mannitol-Specific Phophoenolpyruvate-Dependent Phosphotransferase System of Escherichia coli J. Biomol. NMR (2004) 30 (4): 461 - 642

Publication List on PubMed