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Post-Doctoral Fellow


Educational Background
Chemistry & Biochemistry

Contact
Email: warintra @ brandeis.edu
Office: Volen 443
Dept. of Biochemistry, MS 009
Brandeis University
415 South Street
Waltham, MA 02454, USA
Phone (Lab): (781) 736-2326
Website:Personal Webpage

Warintra Pitsawong


Research Interests

I received my Ph.D. in Chemistry from University of Kentucky in 2014 (research advisor: Prof. Anne-Frances Miller). My Ph.D. research projects include:

  1. Use of pre-steady- state and steady-state kinetics, kinetic isotope effect (primary and solvent KIE), numerical simulation and 2D NMR to understand the unusual broad substrate repertoire of nitroreductase.
  2. Integrated computational approach (QM/MM) to the analysis of 15 N and 13 C NMR in active site of flavodoxin.
  3. 19 F NMR study on substrate activation by tuning of pKas in flavin-dependent aromatic hydroxylases.
I joined the Kern lab in 2015 and am currently working on the rapid kinetic of drugs binding to Aurora A kinase and the evolutionary studies on circadian rhythm in cyanobacterium.


Publications

Pitsawong, W., Sucharitakul, J., Prongjit, M., Tan, T-C., Spadiut, O., Haltrich, D., Divne, C., and Chaiyen, P. (2010) A Conserved active-Site threonine is important for both sugar and flavin oxidation of pyranose 2-oxidase. J. Biol. Chem. 285, 9697- 705.

Tan, T-C., Pitsawong, W., Wongnate, T., Spadiut, O., Haltrich, D., Chaiyen, P., and Divne, C. (2010) H-bonding and positive charge at the N(5)/O(4) locus are critical for covalent flavin attachment in trametes pyranose 2-oxidase. J. Mol. Biol. 402, 578-594.

Tinikul, R.*, Pitsawong, W.*, Sucharitakul, J., Nijvipakul, S., Ballou, DP., and Chaiyen, P. (2013) The transfer of reduced flavin mononucleotide from LuxG oxidoreductase to luciferase occurs via free diffusion. Biochemistry 52, 6834-6843. (*Equal contribution)

Pitsawong, W., Hoben JP., and Miller, AF. (2014) Understanding and exploiting the broad substrate repertoire of nitroreductase via kinetic mechanism. J. Biol. Chem. 289, 15203-14.

Pitsawong, W., Haynes, CA, Koder, RL., Rodgers, DW., and Miller, AF. (2017) Distinct hydride transfers for different substrates in a promiscuous enzyme: nitroreductase Structure 25, 978-987.