| Thu 5/30/13 12 noon | Abelson 229 |
| MRSEC Seminar |
| Cristian Staii (Tufts University) |
| Cytoskeletal dynamics of living neurons measured by combined fluorescence and atomic force microscopy |
Detailed knowledge of mechanical parameters such as cell elasticity, stiffness of the growth substrate, or traction stresses generated during axonal extensions is essential for understanding the mechanisms that control neuronal growth. Here I present results obtained in my research group, which combine Atomic Force Microscopy and Fluorescence Microscopy measurements to produce systematic, high-resolution elasticity maps for different types of live neuronal cells cultured on glass or biopolymer-based substrates. We measure how the stiffness of neurons changes both during neurite outgrowth and upon chemical modification (disruption of the cytoskeleton) of the cell. We find a reversible local stiffening of the cell during growth, and show that the increase in local elastic modulus is primarily due to the formation of microtubules in the cell soma. We also report a reversible shift in the elastic modulus of the cortical neurons cytoskeleton with temperature, from tubulin dominated regions at 37C to actin dominated regions at 25C. We demonstrate that the dominant mechanism by which the elasticity of the neuronal soma changes in response to temperature is the contractile stiffening of the actin component of the cytoskeleton induced by the activity of myosin II motors. |
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| Hosted by Seth Fraden |
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| Fri 5/31/13 9 am | Gerstenzang 122 |
| Chemistry Seminar |
| Klaus Schmidt-Rohr (Iowa State University) |
| NMR and SAXS Analysis of Complex Materials: Nanodiamond, Bone and the Nafion Fuel Cell Membrane |
| Hosted by Judith Herzfeld |
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| Fri 5/31/13 12:30 pm | Shapiro Campus Center Auditorium |
| Special Seminar (James Haber 70th Birthday Celebration) |
| Fred Alt (Boston Children's Hospital / HHMI) |
| Mechanisms of programmed DNA rearrangements and chromosomal translocations in the immune system |
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| Fri 5/31/13 2:15 pm | Shapiro Campus Center Auditorium |
| Special Seminar (James Haber 70th Birthday Celebration) |
| Gregory Ira (Baylor College of Medicine) |
| DNA helicase Pif1 functions with DNA Polymerase in recombination-associated DNA synthesis |
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| Fri 5/31/13 2:40 pm | Shapiro Campus Center Auditorium |
| Special Seminar (James Haber 70th Birthday Celebration) |
| Anna Malkova (IUPUI) |
| Genetic instabilities resulting from break-induced replication |
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| Fri 5/31/13 3:05 pm | Shapiro Campus Center Auditorium |
| Special Seminar (James Haber 70th Birthday Celebration) |
| Ranjith Prasad Anand (Haber Lab) |
| Investigating the properties of a repair replication fork in budding yeast |
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| Fri 5/31/13 4 pm | Shapiro Campus Center Auditorium |
| Special Seminar (James Haber 70th Birthday Celebration) |
| Sang Eun Lee (UT Health Science Ctr, San Antonio) |
| Error prone repair of DNA breaks |
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| Fri 5/31/13 4:25 pm | Shapiro Campus Center Auditorium |
| Special Seminar (James Haber 70th Birthday Celebration) |
| Xiaohua Wu (Scripps) |
| DNA double-strand break repair in mammalian cells |
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| Fri 5/31/13 4:50 pm | Shapiro Campus Center Auditorium |
| Special Seminar (James Haber 70th Birthday Celebration) |
| Marco Foiani (IFOM, Univ. Mian) |
| ATR-mediated control of chromosome integrity |
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| Fri 5/31/13 5:15 pm | Shapiro Campus Center Auditorium |
| Special Seminar (James Haber 70th Birthday Celebration) |
| Nevan Krogan (UCSF) |
| Working with Jim to bridge systems and mechanism |
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| Sat 6/1/13 9 am | Shapiro Campus Center Auditorium |
| Special Seminar (James Haber 70th Birthday Celebration) |
| Scott Keeney (Memorial Sloan-Kettering) |
| ZwiMMing in circles: Controlling DSB numbers when downstream is also upstream |
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| Sat 6/1/13 9:25 am | Shapiro Campus Center Auditorium |
| Special Seminar (James Haber 70th Birthday Celebration) |
| Michael Lichten (Center for Cancer Research, NCI) |
| Three decades of real-time meiotic recombination |
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| Sat 6/1/13 9:50 am | Shapiro Campus Center Auditorium |
| Special Seminar (James Haber 70th Birthday Celebration) |
| Monica Colaiacovo (Harvard Medical School) |
| The many routes to aneuploidy: mechanistic insights from C. elegans |
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| Sat 6/1/13 10:15 am | Shapiro Campus Center Auditorium |
| Special Seminar (James Haber 70th Birthday Celebration) |
| Charles White (CNRS) |
| Rad51 family proteins and recombination in Arabidopsis |
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| Sat 6/1/13 11:10 am | Shapiro Campus Center Auditorium |
| Special Seminar (James Haber 70th Birthday Celebration) |
| Sue Lovett (Brandeis) |
| Template-switching in genomic evolution |
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| Sat 6/1/13 11:35 am | Shapiro Campus Center Auditorium |
| Special Seminar (James Haber 70th Birthday Celebration) |
| Giovanni Bosco (Dartmouth) |
| Condensin mediated interphase chromosome compaction and chromatin tethers modulate nuclear architecture |
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| Sat 6/1/13 12 noon | Shapiro Campus Center Auditorium |
| Special Seminar (James Haber 70th Birthday Celebration) |
| Vinay Eapen (Haber Lab, Brandeis) |
| Crosstalk between DNA damage signalling and the autophagy pathway |
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| Sat 6/1/13 1:30 pm | Shapiro Campus Center Auditorium |
| Special Seminar (James Haber 70th Birthday Celebration) |
| Ed Louis (Univ. Leicester) |
| Exploring natural genetic variation in yeast underlying the genetic architecture of complex traits |
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| Sat 6/1/13 1:55 pm | Shapiro Campus Center Auditorium |
| Special Seminar (James Haber 70th Birthday Celebration) |
| Anne Plessis (Institut Jacques Monod) |
| Cell signalling, protein trafficking and wing patterning in Drosophila |
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| Sat 6/1/13 2:20 pm | Shapiro Campus Center Auditorium |
| Special Seminar (James Haber 70th Birthday Celebration) |
| Bernadette Connolly (Univ. Aberdeen) |
| Spliced leader trans-splicing in nematodes |
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| Sat 6/1/13 2:45 pm | Shapiro Campus Center Auditorium |
| Special Seminar (James Haber 70th Birthday Celebration) |
| John Lydeard (Harvard Medical School) |
| Identifying Cul4 substrates and gaining functional insight through proteomics |
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| Sat 6/1/13 3:40 pm | Shapiro Campus Center Auditorium |
| Special Seminar (James Haber 70th Birthday Celebration) |
| Lance Davidow (Harvard Stem Cell Institute) |
| Chemical biology cell-based screening for neurobiological diseases |
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| Sat 6/1/13 4:05 pm | Shapiro Campus Center Auditorium |
| Special Seminar (James Haber 70th Birthday Celebration) |
| Ellen Kraig (UT Health Science Center San Antonio) |
| Aging, but aren't we all? |
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| Sat 6/1/13 4:30 pm | Shapiro Campus Center Auditorium |
| Special Seminar (James Haber 70th Birthday Celebration) |
| Jim Haber (Brandeis) |
| TBA |
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| Thu 6/6/13 12 noon | Abelson 229 |
| MRSEC Seminar |
| Mo Khalil (Boston University) |
| TBA |
| Hosted by Seth Fraden |
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| Thu 6/6/13 12 noon | Gerstenzang 121 |
| Summer Pizza Talk |
| Markus Gruetter (Institute of Biochemistry, University of Zurich) |
| Structure-function relationships in heterodimeric ABC exporters |
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| Tue 6/11/13 12 noon | Abelson 229 |
| MRSEC Seminar |
| Cristian Micheletti (International School for Advanced Studies (SISSA)) |
| TBA |
| Hosted by M. Hagan |
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Science at Brandeis